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Effects of hns mutations on fimA promoter inversiona
| H-NS derivative | Inversion phenotype |
|---|---|
| pTHK116-7 (wild-type hns) | Red and white (normal) |
| pACYC184 (vector) | Pink (rapid) |
| M1I | Pink |
| A18E | Pink |
| L26P | Pink |
| L65P | Pink |
| R93H | Red and white |
| T108I | Red and white |
| G111S | Red and white |
| N67 | Pink |
| N93 | Pink |
| N123 | Pink |
All plasmids were transformed into AL29 (fimA′lacZYA-kan, hns2-tetR) and grown at 37°C on lactose MacConkey indicator medium. Individual colonies were picked and restruck to verify phenotypes.
