Figure 2.

Conjugation efficiencies of pKSR100 and pAPR100 among different donors, recipients and media conditions. Different donor strains included native S. flexneri hosts (orange) and clinical isolate S. sonnei 216 as an isogenic donor (blue) while the recipient strains were either E. coli MG1655 (in grey) or S. sonnei 216 (in blue). Results for each plasmid are coloured according to the inlaid key. Results from liquid media are shown upper while solid is shown lower. Each box plot represents the combined results of all the time points used in LM models, where there are 12 replicates (four biological, three technical) for each time point. The asterisks denote significance as determined LMs. The p-values for each of the panels from left to right are as follows: top row, p = 0.043, p < 0.000; bottom row, p = 0.026, p = 0.025. Note: conjugations between S. flexneri donors and S. sonnei recipients are not shown here as no transconjugants were recovered until 3 h after mating, making it difficult to control for the growth rates of strains involved. Therefore, our main claims are based on liquid media conjugations where first transconjugants were recovered within 15 min of mating. (Online version in colour.)