Table 1.

The impact of pKSR100 and pAPR100 on antimicrobial resistance phenotypes.

					MIC values by plasmid and hosta
antimicrobial resistance			gene present in:		neither plasmid		with pKSR100			with pAPR100
class	gene	antibiotic	pKSR100	pAPR100	E. coli	S. sonnei 216	S. flexneri (native)	E. coli + pKSR100	S. sonnei 216 + pKSR100	S. flexneri (native)	E. coli + pAPR100	S. sonnei 216 + pAPR100
macrolide	mph(A)	azithromycin	√	√	4	4	>256	>256	>256	48	64/32	64
macrolide	erm(B)	clindamycin	√		>256	48	>256	>256	>256	>256	>256	>256
sulfonamide	sul1	sulfamethoxazole	√		3	8	>1024	12	>1024	4	3	8
trimethoprim	dfrA17	trimethoprim	√		0.125	>32	>32	>32	>32	>32	0.125	>32
penicillins	blaTEM1b/1a	ampicillin	√	√	2	4	>256	>256	>256	>256	>256	>256
aminoglycoside	aadA5	streptomycin	√		1	16	48/64	4	24	128	1	16
tetracycline	tetA	tetracycline		√	0.75	0.75	0.75	0.75	0.75	64	48	32
fluoroquinolone		ciprofloxacin			0.006	0.004	0.006	0.006	0.004	0.004	0.006	0.006
cephalosporin		cefotaxime			0.016	<0.016	0.023	0.016	<0.016	0.016	0.016	<0.016

^aMIC values in bold represent a change of four-fold or more in MIC when the relevant plasmid is present.