Figure 5. TREM1 expression is associated with the clinical outcome of healing in DFUs.
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AqPCR of TREM1 and genes involved in neutrophil recruitment demonstrate increased expression in healing DFUs compared to nonhealing DFUs (n = 5 healing and n = 6 nonhealing). Data presented as mean ± SD. *P < 0.05. **P < 0.01 (two‐tailed unpaired Student's t‐test).
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BRepresentative images of healing and nonhealing DFUs show basal keratin marker K5 and TREM1 and corresponding quantification from healing (n = 4) and nonhealing (n = 4) is shown in the graph. Data presented as mean ± SEM. *P < 0.05 (two‐tailed unpaired Student's t‐test). (Scale bar: 50 μm).
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CRepresentative images of cit‐H3 immunohistochemistry show increase staining in nonhealing DFUs when compared to healing DFUs, which was confirmed by corresponding quantification from healing (n = 3) and nonhealing (n = 3), shown in the graph. Data presented as mean ± SD. *P < 0.05 (two‐tailed unpaired Student's t‐test). (Scale bar: 50 μm).