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. 2022 Jun 15;23(8):e52280. doi: 10.15252/embr.202052280

Figure EV1. Activation of PPARα suppresses iron overload‐induced Ferroptosis in vivo .

Figure EV1

  1. Serum ALT and AST levels was measured in 8‐week‐old WT mice that were received intraperitoneal injections of dextriferron with or without GW7647 treatment.
  2. Liver sections were obtained from the indicated mice and stained with H&E. All scale bars are 50 μm.
  3. Liver sections were obtained from the indicated mice and stained with TUNEL. All scale bars are 20 μm.
  4. Hepatic and serum HMGB1 content were measured in the indicated mice.
  5. Hepatic Gpx4 mRNA levels were measured in the indicated mice.
  6. Hepatic MDA content was measured in 8‐week‐old WT mice that were received intraperitoneal injections of dextriferron with or without GW7647 treatment.
  7. Hepatic iron content was measured in 8‐week‐old WT mice that were received intraperitoneal injections of dextriferron with or without GW7647 treatment.

Data information: In (A–G), n = 3–5 mice/group. mRNA levels were normalized to 36B4 and are expressed relative to the mean value of the WT group; Data are presented as means ± SD. *P < 0.05, **P < 0.01, determined by ANOVA.