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. 2022 Jun 15;23(8):e52280. doi: 10.15252/embr.202052280

Figure EV2. Activated PPARα inhibited Ferroptosis caused by iron overload.

Figure EV2

  1. Hepatic FSP1 mRNA levels were measured in the indicated mice.
  2. Hepatic MDM2 and MDMX mRNA levels were measured in the indicated mice.
  3. MDM2, MDMX, CPT1 and GPX4 mRNA levels were measured in the Hep1‐6 cells after MDM2 or MDMX knockdown.
  4. Hepatic NRF2 mRNA levels, hepatic HO1 mRNA levels, and hepatic NQO1 mRNA levels were measured in the indicated mice.
  5. Liver sections were obtained from the indicated mice and stained with Prussian blue All scale bars are 50 μm.
  6. Hepatic iron content was measured in 8‐week‐old WT, PPARα−/− mice that were fed a HID or received intraperitoneal injections of dextriferron; n = 3–5 mice/group.

Data information: In (A, B, D‐E), n = 3–5 mice/group. mRNA levels were normalized to 36B4 and are expressed relative to the mean value of the WT group; Data are presented as means ± SD. *P < 0.05, **P < 0.01, determined by ANOVA.