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. 2022 Jun 15;23(8):e52280. doi: 10.15252/embr.202052280

Figure 3. PPARα deficiency influences iron metabolism in the liver.

Figure 3

  1. Fluorescent images of labile Fe2+ in hep1‐6 cells. Hep1‐6 cells were incubated with control, 20 μM probe1 for 30 min; 20 μM Cu2+ for 30 min then 20 μM probe1 for another 30 min; 20 μM Fe3+ for 30 min then 20 μM probe1 for another 30 min; 20 μM Fe2+ for 30 min then 20 μM probe1 for another 30 min; All scale bars are 20 μm. n = 3 biological replicates.
  2. Liver sections were obtained from the indicated mice and stained with probe1. All scale bars are 20 μm. n = 3 biological replicates.
  3. Hepatic Acox1 and TRF mRNA levels were measured in the WT and PPARα−/− mice with or without GW7647 treatment. n = 3–5 mice/group.
  4. TRF protein was measured in liver of WT and PPARα−/− mice fed blank solvent or GW7647. n = 4 biological replicates.
  5. Hepatic TRF mRNA levels were measured in the indicated mice. n = 3–5 mice/group.
  6. Hepatic and serum TRF content were measured in the indicated mice. n = 3–5 mice/group.

Data information: mRNA levels were normalized to 36B4 and are expressed relative to the mean value of the WT group. Data are presented as means ± SD. *P < 0.05, **P < 0.01, determined by ANOVA.