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. 2022 Jun 15;23(8):e52280. doi: 10.15252/embr.202052280

Figure EV4. PPARα deletion increases ferroptosis caused by iron overload.

Figure EV4

  1. Hepatic iron content was measured in 8‐week‐old WT, PPARα−/− mice that received intraperitoneal injections of dextriferron or saline.
  2. Serum ALT and AST levels was measured in 8‐week‐old WT, PPARα−/− mice that received intraperitoneal injections of dextriferron or saline.
  3. Liver sections were obtained from the indicated mice and stained with H&E. All scale bars are 50 μm. n = 3 biological replicates.
  4. Hepatic MDA content was measured in 8‐week‐old WT, PPARα−/− mice that received intraperitoneal injections of dextriferron or saline.
  5. Measurement of intracellular ROS levels by fluorescent probe DCFH‐DA, and the fluorescence intensity of ROS was calculated. All scale bars are 20 μm. n = 3 biological replicates.
  6. Hepatic Gpx4 mRNA levels were measured in the indicated mice.
  7. Hepatic Gpx4 mRNA levels were measured in the mice that were fed a HID with or without Gpx4‐AAV treatment.

Data information: In (A–G), n = 3–5 mice/group. mRNA levels were normalized to 36B4 and are expressed relative to the mean value of the WT group; Data are presented as means ± SD. *P < 0.05, **P < 0.01, determined by ANOVA.