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. 2022 Jun 29;23(8):e54265. doi: 10.15252/embr.202154265

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© 2022 The Authors. Published under the terms of the CC BY 4.0 license.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure EV4 — A
Immunofluorescence staining of vimentin in indicated cell lines. F‐actin was stained with phalloidin, and nucleus was stained with DAPI. Scale bar, 10 μm. Data shown are representative of three independent experiments.

B
Fractionation of soluble and insoluble V5‐vimentin. CL1‐0 cells were transiently expressed with V5‐vimentin wild‐type, 4RK and 4RF mutants for 48 h. The cells were fractionated to supernatants (S) and pellets (P). After pellets were resolved by sonication, equal portions of lysates were subjected to immunoblotting. The ratio of V5‐vimentin in the supernatant and pellet fractions was measured by ImageJ software. Data shown are representative of three independent experiments.