Figure 1. Identification of downstream candidate target genes of Bcl11a in superficial cortical layers at early postnatal development.

• A
  Cortical layers 2–4 were isolated by laser microdissection from Bcl11a ^F/F ;Emx1 ^IRESCre and control neocortex (n = 4).
• B
  Gene expression was compared using microarrays. From a set of 137 differentially expressed (DE) genes, candidate targets were selected based on gene ontology (GO) and PubMed analyses and verified by quantitative real‐time PCR and RNA in situ hybridization.
• C
  Volcano plot showing DE genes (red). Those not significantly changed (fold change < 1.5; P > 0.05) are shaded black. Bcl11a and Bcl6 are highlighted in green.
• D
  Relative Bcl6 mRNA expression level determined by quantitative real‐time PCR is decreased in laser‐microdissected cortical tissue of P2 Bcl11a ^F/F ;Emx1 ^IRESCre compared with control brains (n = 4). Results are expressed as mean ± s.e.m.; Student's t‐test; **P < 0.01.
• E
  RNA in situ hybridization showing downregulation of Bcl6 expression in Bcl11a ^F/F ;Emx1 ^IRESCre compared with control neocortex at E17.5, P0, P2, and P4.

Data information: Scale bars, 100 μm (A) and 50 μm (E).