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. 2022 Jun 29;23(8):e54104. doi: 10.15252/embr.202154104

Figure EV3. Selected candidate downstream target genes of Bcl11a in superficial cortical layers at early postnatal development.

Figure EV3

  • A
    Volcano plot showing differentially expressed (DE) genes in laser‐microdissected cortical layers 2–4 of Bcl11a F/F ;Emx1 IRESCre neocortex compared with controls. DE genes not significantly changed (fold change < 1.5; P > 0.05) are shaded black. Cdh6, Cdh12, Efna5, Pcdh9, Cdh13, Flrt2, Flrt3, and Slit2 are highlighted in green.
  • B
    Relative mRNA expression levels of selected DE genes determined by quantitative real‐time PCR in laser‐microdissected cortical tissue of P2 Bcl11a F/F ;Emx1 IRESCre and control brains (n = 4). Graph represents mean ± s.e.m.; Student's t‐test; *P < 0.05; **P < 0.01; ***P < 0.01.
  • C
    RNA in situ hybridization of selected DE genes with decreased expression in P2 Bcl11a F/F ;Emx1 IRESCre compared with control neocortex.
  • D
    RNA in situ hybridization of selected DE genes with increased expression in P2 Bcl11a F/F ;Emx1 IRESCre compared with control neocortex.

Data information: Scale bar, 50 μm.