Figure EV4. Relative Bcl6 expression is unchanged in deep cortical layers of Bcl11a ^F/F ;Emx1 ^IRESCre compared with control neocortex.

• A
  Immunohistochemistry of Bcl6 (red), Bcl11b (green), and Tbr1 (blue) in P2 Bcl11a ^F/F ;Emx1 ^IRESCre and control neocortex. Bcl6 and Tbr1 expressions are downregulated, while Bcl11b expression is upregulated in Bcl11a ^F/F ;Emx1 ^IRESCre compared with control neocortex. Nuclei are stained with Dapi (white).
• B
  Relative quantification of Bcl6⁺, Bcl11b⁺, and Tbr1⁺ cells in Bcl11a ^F/F ;Emx1 ^IRESCre and control neocortex (n = 4).
• C
  Numbers of Bcl11b⁺ or Tbr1⁺ cells that coexpress Bcl6 are normal in Bcl11a ^F/F ;Emx1 ^IRESCre compared with control neocortex (n = 4).

Data information: All graphs represent mean ± s.e.m.; Student's t‐test; **P < 0.01; ***P < 0.001. Scale bar, 50 μm.