Figure 5. Postnatal developmental cell death is increased in Bcl6 ^F/F ;Nex ^Cre neocortex.

• A
  Immunohistochemistry of cleaved caspase 3 (CC3) shows that the number of CC3⁺ cells (marked by black arrowheads) is increased in P5 Bcl6 ^F/F ;Nex ^Cre compared with control neocortex. Insets are enlargements of the boxed areas in corresponding panels.
• B
  Relative Bcl6 mRNA expression level determined by quantitative real‐time PCR using primers targeting a region of exon 8 is decreased in P0 Bcl6 ^F/F ;Nex ^Cre compared with control brains (n = 4). Results are expressed as mean ± s.e.m.; Student's t‐test; ***P < 0.001.
• C
  Quantification of the experiment shown in (A) (n = 3). Results are expressed as mean ± s.e.m.; Student's t‐test; *P < 0.05.
• D
  Quantification of neocortical area in P5 Bcl6 ^F/F ;Nex ^Cre and control brains (n = 3). Results are expressed as mean ± s.e.m.; Student's t‐test; **P < 0.01.
• E
  Heat map showing differentially expressed genes in laser‐microdissected superficial cortical layers of P5 Bcl6 ^F/F ;Nex ^Cre compared with control brains (n = 4).
• F
  Relative Foxo1 mRNA expression level determined by quantitative real‐time PCR is increased in laser‐microdissected superficial cortical layers of P5 Bcl6 ^F/F ;Nex ^Cre compared with control brains (n = 4). Results are expressed as mean ± s.e.m.; Student's t‐test; **P < 0.01.
• G
  RNA in situ hybridization showing upregulation of Foxo1 expression in P5 Bcl6 ^F/F ;Nex ^Cre compared with control neocortex.
• H
  Schematic representation of the experimental approach. Embryos are electroporated at E15.5 and sacrificed at P5.
• I
  DNA plasmids used in the experiment shown in (J and K).
• J
  Immunohistochemistry of electroporated P5 Bcl11a ^F/F neurons in superficial cortical layers with GFP (green) and cleaved caspase 3 (CC3, magenta) antibodies. Electroporation of CAG‐Cre ^GFP plasmid together with Foxo1‐shRNA ^GFP #4 into Bcl11a ^F/F neocortex reduces the number of CC3⁺ cells to control levels. White and black arrowheads indicate GFP⁺ CC3⁺ and GFP⁺ cells, respectively.
• K
  Quantification of the experiment shown in (J) (n = 4, CAG‐Ctl ^GFP /Ctl‐shRNA ^GFP ; n = 3, CAG‐Cre ^GFP /Ctl‐shRNA ^GFP ; n = 5, CAG‐Cre ^GFP /Foxo1‐shRNA ^GFP #4). Results are expressed as mean ± s.e.m.; one‐way ANOVA followed by Tukey's post‐hoc test; *P < 0.05.

Data information: Scale bars, 500 μm (A), 50 μm (G, J).