Figure EV5. Increased cell death in Bcl11a ^F/F ;Nex ^Cre and overlapping differentially expressed genes in upper cortical layers of Bcl11a ^F/F ;Emx1 ^IRESCre and Bcl6 ^F/F ;Nex ^Cre compared with controls.

• A
  Immunohistochemistry of cleaved caspase 3 (CC3) shows that the number of CC3⁺ cells is increased in P5 Bcl11a ^F/F ;Nex ^Cre compared with control neocortex.
• B
  Quantification of the experiment shown in (A).
• C
  Venn diagram showing the number of overlapping differentially expressed (DE) genes in P2 Bcl11a ^F/F ;Emx1 ^IRESCre and P5 Bcl6 ^F/F ;Nex ^Cre upper cortical layers.
• D, E
  Relative mRNA expression levels of the overlapping DE genes Foxo1, Ldb2, and Socs2 as well as Trp53 determined by quantitative real‐time PCR in laser‐microdissected cortical tissue of P2 Bcl11a ^F/F ;Emx1 ^IRESCre compared with control brains (D) or P5 Bcl6 ^F/F ;Nex ^Cre compared with control brains (E).

Data information: All graphs represent mean ± s.e.m.; n = 4; Student's t‐test; *P < 0.05; **P < 0.01; ***P < 0.001. Scale bar, 500 μm.