Figure EV1. DcR1 is expressed on CD4 T cells.

Primary enriched CD4 T cells were stimulated for 3 days and then infected with the HIV strain NL4‐3. Four days post‐infection cells were labeled with LIVE/DEAD Fixable Near‐IR Stain, followed by incubation with αCD4‐APC, αp24‐FITC, goat anti‐human DcR1, and then labeled with anti‐goat‐PE. Expression was measured as median fluorescence intensity (MdFI) by flow cytometry.

1. Representative flow cytometry histogram of DcR1 expression on uninfected (HIV⁻: CD4⁺/p24⁻) and infected (HIV⁺: CD4⁻/p24⁺) cells in comparison to the anti‐goat‐PE control (grey) or FMO control (dashed line).
2. Cumulative data of DcR1 expression displayed as MdFI. Data points represent the mean of two technical replicates per condition of three different donors (n = 3). Bar graphs show the median. Error bars show the IQR.

Source data are available online for this figure.