Figure 7. TRAIL contributes to NK‐cell‐mediated killing of target cells.

Lysis of different target cells in co‐culture with NK cells was quantified in various cytotoxicity assays.

1. Left panel: Representative contour plots showing depletion of 721.221 target cells in the presence of NK cells. Middle panel: Percentage of target cells remaining (y‐axis) after co‐culture with NK cells in the presence of either αTRAIL or isotype control, in reference to target cells kept alone. Right panel: Box plots displaying difference in target cells remaining (y‐axis) between αTRAIL and isotype conditions displayed as p.p. (n = 12 different donors). Each data point represents the mean of at least two technical replicates.
2. Left panel: Representative contour plots showing the percentage of .221‐DR4/5KO (control) and .221‐Cas9 cells (target) in the presence or absence of NK cells. Middle panel: Ratio between .221‐DR4/5KO and .221‐Cas9 cells (y‐axis) in the presence or absence of NK cells. Right panel: Specific lysis of .221‐Cas9 cells displayed as percent (n = 12 different donors). Each data point represents the mean of at least three technical replicates.
3. Left panel: Representative contour plots showing the percentage of Raji‐pSIP (control) and Raji‐DR5⁺⁺ (target) in the presence or absence of NK cells. Middle panel: Ratio between Raji‐pSIP and Raji‐DR5⁺⁺ (y‐axis) in the presence or absence of NK cells. Right panel: Specific lysis of Raji‐DR5⁺⁺ cells displayed as % (n = 12 different donors). Each data point represents the mean of at least three technical replicates.

Data information: Wilcoxon signed‐rank test. Experiments were performed in four batches with three different donors each. “No NK” control samples served as a reference for all donors in each batch. Lines connect each data value of the NK cell condition with their designated “No NK” control. Box plots represent the median and 25%/75% percentile. Whiskers indicate minimum and maximum data points.

Source data are available online for this figure.