Figure EV2. MiR‐324‐5p does not regulate the expression of RIPK1 or RIPK3.

• A
  HT‐29 cells were transfected with NC, siRIPK1, or miR‐324‐5p mimics for 48h. qPCR analysis for the expression of RIPK1 (left) and RT‐PCR analysis of RIPK1 and GAPDH (right). Quantification of RIPK1 normalized to GAPDH (under the band of RIPK1).
• B
  HT‐29 cells were transfected with NC, siRIPK3, or miR‐324‐5p mimics for 48h. qPCR analysis for the expression of RIPK3 (left) and RT‐PCR analysis of RIPK3 and GAPDH (right). Quantification of RIPK3 normalized to GAPDH (under the band of RIPK3).

Data information: Data are represented as the means ± SD of three biological replicates. Statistical analyses were performed using unpaired Student's t‐test. All experiments were performed at least three times, and representative data are shown.