Table 1.

Measured velocities and run‐lengths of all KIF5A constructs.

Construct	Velocity (mean ± SD) (μm/s)	Processivity (lifetime [95% CI]) (μm)
KIF5A WT‐GFP	0.45 ± 0.11	0.60 [0.51, 0.71]
∆Exon27‐GFP	0.66 ± 0.10	5.47 [3.84, 7.80]
∆C‐GFP	0.85 ± 0.07	1.63 [1.47, 1.81]
K998‐GFP	0.70 ± 0.11	2.07 [1.72, 2.50]
KIF5A WT‐GFP/∆Exon27‐mCherry (KIF5A WT‐GFP a )	0.62 ± 0.10	3.95 [2.81, 5.53]
KIF5A WT‐GFP/∆Exon27‐mCherry (∆Exon27‐mCherry a )	0.62 ± 0.10	4.49 [3.64, 5.63]

The velocity data were fit with Gaussian distribution, and the run‐length data were fit with an exponential decay function.

^a Denotes the fluorophore with which KIF5A movement was tracked.