Table 1.
Construct | Velocity (mean ± SD) (μm/s) | Processivity (lifetime [95% CI]) (μm) |
---|---|---|
KIF5A WT‐GFP | 0.45 ± 0.11 | 0.60 [0.51, 0.71] |
∆Exon27‐GFP | 0.66 ± 0.10 | 5.47 [3.84, 7.80] |
∆C‐GFP | 0.85 ± 0.07 | 1.63 [1.47, 1.81] |
K998‐GFP | 0.70 ± 0.11 | 2.07 [1.72, 2.50] |
KIF5A WT‐GFP/∆Exon27‐mCherry (KIF5A WT‐GFP a ) | 0.62 ± 0.10 | 3.95 [2.81, 5.53] |
KIF5A WT‐GFP/∆Exon27‐mCherry (∆Exon27‐mCherry a ) | 0.62 ± 0.10 | 4.49 [3.64, 5.63] |
The velocity data were fit with Gaussian distribution, and the run‐length data were fit with an exponential decay function.
Denotes the fluorophore with which KIF5A movement was tracked.