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. 2022 Jul 26;2022:3695848. doi: 10.1155/2022/3695848

Figure 5.

Figure 5

HuAFSC-exosomes rely on hsa-miR-369-3p to inhibit the expression of YAF2 in OGCs to achieve therapeutic effects in POF mice. (a) Experimental design. (b) Ovarian pathological identification was carried out by hematoxylin and eosin staining (magnification 200x). (c) Immunofluorescence analysis showed that the Dil-labelled HuAFSC-exosomes were located in the OGCs of mice. (d) Ovarian weight was higher in the HuAFSC-exosome-treated mice than in the saline-treated mice (P < 0.01 vs. POF + saline-treated mice; t-test; n = 8). (e) Normal follicle count was higher in the HuAFSC-exosome group than in the saline group, and fewer atretic follicles were present in the HuAFSC-exosome-treated mice than in the saline-treated mice (P < 0.01 vs. POF + saline-treated mice; t-test; n = 8). (f) qRT-PCR results showed that the mRNA expression levels of Ki67, Bcl2, and miR-369-3p were significantly higher in the mOGCs of the HuAFSC-exosome-treated mice than in the OGCs of the saline-treated mice, and the mRNA expression levels of BAX, P53, PDCD5, and YAF2 were significantly lower in the mOGCs of the HuAFSC-exosome-treated mice than in the OGCs of the saline-treated mice (P < 0.01 vs. POF + saline-treated mice; t-test; n = 3).