Figure 5.

Fruit shape phenotypes of BZR1.7 knockout lines. a Two sgRNA targets (red font) were designed in exon 1 of the BZR1.7 gene. Black arrows indicate the locations of the primers used to evaluate mutation types in transgenic plants created using the CRISPR/Cas9 technique. b Fruit shape index in the BZR1.7 CR lines and the WT control. c Genomic DNA sequences of target regions from the WT plant (BZR1.7) and three CR lines from the T1 generation. Red letters are the sgRNA target sequences and black boxes represent the protospacer-adjacent motif (PAM) sequences. Black dashed lines represent ellipsis sequences. Green dashed lines represent genomic DNA deletions. CRI, CRISPR/Cas9. d Phenotype of red ripe fruit from the three BZR1.7 CR lines and WT control.