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. 2022 Aug 2;22:843. doi: 10.1186/s12885-022-09808-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Ionizing radiation inhibits proliferation linked with increased cytotoxicity and apoptosis but only in U251-MG GB cells. For these experiments, GB cells were cultivated at 21% or 3% oxygen in non-irradiated condition (0) or 3 days after an irradiation of 4 or 16 Gy. Numbers of nuclei of U251-MG (A) and U87-MG (B) GB cells are expressed as mean ± Standard Error of the Mean (S.E.M.) (n = 3). Measure of Lactate Dehydrogenase (LDH) release into cell culture medium of U251-MG (C) and U87-MG (D) GB was assessed. Cytotoxicity is expressed as mean percentage ± SEM (n = 3) of the total amount of LDH released from cells and relative to GB cells treated 0.1% Triton X-100, given the arbitrary percentage of 100. DEVD-AMC caspase-3 activity in U251-MG (E) and U87-MG (F) GB cells is expressed as mean arbitrary units (A.U.) of fluorescence per 30 µg of proteins ± SEM (n = 3). One-way ANOVA test was performed between non-irradiated (0 Gy) or irradiated (4 or 16 Gy) conditions (^*, p-value ≤ 0.05; ^**, p-value ≤ 0.01; ^***, p-value ≤ 0.001; ^****, p-value ≤ 0.0001)