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. Author manuscript; available in PMC: 2023 Jul 20.
Published in final edited form as: J Am Chem Soc. 2022 Jul 8;144(28):12756–12768. doi: 10.1021/jacs.2c03324

Figure 5.

Figure 5.

Activity of l-EP against E. coli CFT073 and the fepC and fes mutants. (A) Representative phase-contrast and fluorescence micrographs of E. coli CFT073, its inner membrane transporter mutant fepC, and its cytoplasmic esterase mutant fes treated with 15 µM l-EP in modified M9 medium for 11 h at 30 °C. Scale bar: 10 mm. (B) Quantification of the l-EP-induced bacterial morphologies in E. coli. (C) Viability of E. coli determined by LIVE/DEAD staining. For morphology and viability quantification, 500–1000 cells were manually counted for each condition from micrographs. For morphology quantification, cell size was measured and grouped by perimeter. Activity of l-EP against the inner membrane transporter mutant fepDG and the cytoplasmic esterase mutant iroD are shown in Figure S14.