Fig. 1.

A ARv7 mRNA and protein levels in 22Rv1 cells after 4 Gy IR treatment. B ARv7 mRNA and protein levels in 22Rv1 xenografts after 4 Gy IR treatment. C C4-2-IRR parental cells and C4-2-IRR-shARv7 cells were cultured with 5 µM Enz for 24 h, then treated with 0-6Gy IR. D The oeARv7 effects on C4-2 cells survival after 0-6 Gy IR using clonogenic assay. E The shARv7 and/or shAR effects on 22Rv1 cells survival after IR using clonogenic assay. F shARv7 effects on DNA damage in C4-2-IRR cells after IR using comet assay. Scale bar = 10 µm. G WB analysis of γ-H2AX in different groups. The oeARv7 can decrease DNA damage repair time in C4-2 cells and shARv7 can increase DNA damage repair time in C4-2-IRR cells. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, compared with the controls. N.S., not significant