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. 2022 Aug 3;41:235. doi: 10.1186/s13046-022-02287-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — A PCR analysis of 20 circRNAs levels in C4-2 parental cells and C4-2-IRR cells. B Knocking down ARv7 with shARv7 in C4-2-IRR cells and PCR analysis of 7 circRNAs levels. C oeARv7 in C4-2 parental cells and PCR analysis of 7 circRNAs levels. D circNHS levels in 22Rv1 cells xenografts after 4 Gy IR treatment. E Schematic diagram of the genomic location and splicing pattern of circNHS. F The existence of circNHS was validated in C4-2 parental cells and 22Rv1 cells by PCR. Divergent primers amplified circNHS from cDNA, but not from genomic DNA (gDNA). GAPDH was used as a negative control. G PCR analysis of RNase R treatment assay to confirm the circNHS formation is a circRNA sequence. H The relative RNA levels of circNHS and NHS were analyzed by RT-qPCR after treatment with Actinomycin D at the indicated time points. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the controls. N.S., not significant