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. 2022 Aug 3;41:235. doi: 10.1186/s13046-022-02287-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — A ChIP assay of the two potential ARE binding sites on NHS promoter. B Co-transfection of wild-type ARE or mutant NHS promoter pGL3-Luciferase constructs into 22Rv1 cells with/without shARv7. Luciferase activity in 22Rv1 cells carried wild-type or mutant NHS promoter plasmids. C shcircNHS effects on C4-2-IRR cells survival after IR using clonogenic assay. D The oecircNHS effects on C4-2 cells survival after IR using clonogenic assay. E WB analysis of γ-H2AX in different groups. The oecircNHS can decrease DNA damage repair time in C4-2 cells and shcircNHS can increase DNA damage repair time in C4-2-IRR cells. F shcircNHS effects on DNA damage in C4-2-IRR cells after IR using comet assay. Scale bar = 10 µm. G The oeARv7 and/or shcircNHS effects on C4-2 cell survival after IR using clonogenic assay H shARv7 and/or oecircNHS effects on C4-2-IRR cells survival after IR using clonogenic assay. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the controls. N.S., not significant