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. Author manuscript; available in PMC: 2022 Aug 3.
Published in final edited form as: Exp Gerontol. 2019 Oct 18;128:110739. doi: 10.1016/j.exger.2019.110739

Fig. 4.

Fig. 4.

Viability and integrity of islets isolated from young and older organ donors. (A) Morphology of islets in bright field. Scale bar: 500 μm. (B) Viability staining of isolated islets between the three groups. Green and red indicate live and dead cells. Scale bar: 500 μm. (C) Analysis of viability revealed that > 41 week-old donors had significantly lower viability (79.5%, n = 3) compared to 21–40 week-old donors (92.5%, p = 0.0275, n = 4) and 10–20 week-old donors (97.8%, p = 0.0053, n = 4). (D) Islet size (area) was measured in the images acquired to assess viability. Islet area in 10–20 week-old donors (11,837 μm2) were significantly larger in comparison to > 41 week-old donors (9035 μm2, p = 0.0399). (E) Representative shape factor of isolated islets calculated by imaging software (cellSens). Higher numbers imply more uniform spherical shape. (F) Analysis of shape factor demonstrated a significant difference in 10–20 week-old donors (0.61) when compared to 21–40 week-old (0.46, p = 0.0007) and > 41 week-old donors (0.48, p = 0.0024). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)