Fig. 4. Native chemical ligation (NCL) to obtain the synthetic Bcr-Abl SH2 domain. (A) Scheme of the NCL between the N- (2) and C-terminal (3) Bcr-Abl SH2 peptides to ligation product 4. (B) Overlay of the chromatograms before (black) and after (red) ligation. A depletion of peptides 2 and 3 is visible while the ligation product 4 emerges. The peak marked with * corresponds to the hydrolysis product of peptide 2.
