Fig. 3. (A) Toehold mediated displacement system using PNA-C1-FAM. When hybridized to the masking sequence, fluorescence is quenched. When a releasing sequence is introduced, the PNA is released, and fluorescence intensity increases. (B–D) % Displacement from PNA-C1-FAM:MS using RS-D, RS-R, and RS-D-m1, respectively, was evaluated in relation to the equivalents of RS added. (B) MS-D. (C) MS-R. (D) MS-D-m1. PNA:MS duplex was used at 3 μM in 1× PBS. Error bars represent standard error (n = 3).
