Fig. 4. (A) Toehold mediated displacement system using PNA-A1-FAM. When hybridized to the masking sequence, fluorescence is quenched. When a releasing sequence is introduced, the PNA is released, and fluorescence intensity increases. Upon release, the amphiphile can then self-assemble. (B) % Displacement from PNA-A1-FAM:MS-D using RS-D was evaluated in relation to the equivalents of RS-D added. (C) % Displacement from PNA-A1-FAM:MS-D using RS-R was evaluated in relation to the equivalents of RS-R added. (D) % Displacement from PNA-C1-FAM:MS-D and PNA-A1-FAM:MS-D using RS-D-sc was evaluated in relation to the equivalents of RS-D-sc added. PNA:MS-D duplex was used at 3 μM in 1× PBS. Error bars represent standard error (n = 3).