FIG. 1.

(A) Gross morphology of neocartilage constructs: All constructs appear homogeneous, flat, and with no visible abnormalities. The ionomycin-treated constructs appear to be the thickest. (B) Histological analysis of neocartilage constructs: Overall cellularity and neotissue composition appears comparable among all groups as observed in Hematoxylin and Eosin staining. Picrosirius Red staining shows a localized distribution in untreated constructs and ouabain-treated constructs display. Safranin O intensity is higher in 4-αPDD and ionomycin-treated constructs. 4-αPDD, 4α-phorbol-12,13-didecanoate. Color images are available online.