Table 1.

Designed primers for genotyping of the studied SNPs

Genes/SNPs	Function of SNPs	Genotyping methods	Primer sequences	Annealing temp (°C)	RE	Product size (bp)
TNFA
rs361525 G>A	Promoteric	RFLP‐PCR	F: ATCTGGAGGAAGCGGTAGTG	55	MspI	G: 132 + 20
			R: AGAAGACCCCCCTCGGAACC			A: 152
IL1RN
rs419598 T>C	Synonymous	RFLP‐PCR	F: TTCCGTCTCTTGAAACTTCTACCT	56	MspI	T: 314
			R: AAAGACCCAACAAGGATTAGGACAT			C: 157
IL6R
rs2228145 A>C	Missense	RFLP‐PCR	F: GTTAAGCTTGTCAAATGGCCTGTT	55	HinfI	A: 258
			R: CAGAGGAGCGTTCCGAAGG			C: 188 + 170
IL6
rs2069827 G>T	Promoteric	ARMS‐PCR	F (G‐allele): CAACTGAGGTCACTGTTTTAGCG	62	‐	G or T: 150
			F (T‐allele): CAACTGAGGTCACTGTTTTAGCT
			R (Common): GACAGCTCTGAGATGGCTTCA

Abbreviations: ARMS‐PCR, amplification refractory mutation system polymerase chain reaction; bp, base pair; F, forward; R, reverse; RE, restriction enzyme; RFLP‐PCR, restriction fragment length polymorphism polymerase chain reaction; SNP, single‐nucleotide polymorphism.