FIG. 3.

Carboxypeptidase A digestion of the C-terminal region of RbsC. Right-side-out vesicles and IOV with RbsC-His-tagged proteins were digested with carboxypeptidase A for various times (30, 60, and 90 min). The digestion products of vesicles were resolved by sodium dodecyl sulfate–12% polyacrylamide gel electrophoresis and immunoblotted with His tag conjugate. The immunoreactive bands were quantified with the ImageQuant program. The white and black bars indicate the percentages of the remaining immunoreactive bands of right-side-out vesicles and IOV, respectively.