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. 2022 Jul 31;14(1):2104087. doi: 10.1080/19490976.2022.2104087

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Cross-fostering of CD1d^−/− mice impacts microbiota composition. (a) Schematic of cross-fostering, breeding and housing. (b) 16S bacterial rRNA sequencing was used to define the microbiota profiles of CD1d^+/+ and CD1d^−/− non-littermate mice prior to cross-fostering (Pre) and CD1d^+/+ and CD1d^−/− littermate mice post-cross-fostering (Post) (n = 6 to 8 mice per group). These groups were separated by principal coordinates PCo1 and PCo2, collectively explaining 83.4% of the total similarity between samples, based on Bray-Curtis distances. (c) Relative abundance (family level) varied between CD1d^+/+ and CD1d^−/− mice pre-cross-fostering, as well as between mice pre- and post-cross-fostering. (d) MetagenomeSeq analysis shows the top 10 most enriched OTUs in CD1d^−/− mice pre- and post-cross-fostering. Log2 fold-change values are shown.