Figure 3.

Increased iNKT cells prevalence in Vα14 Tg mice does not impact microbiota composition. (a, b) Frequency of TCRβ⁺ PBS57-CD1d tetramer-positive iNKT cells out of live CD19⁻ lymphocytes and subset distribution of iNKT cell subsets in the mesenteric lymph nodes (mLN, A) and colon (b) of WT and Vα14 Tg mice. Data shows individual and mean values ± s.e.m. (n = 3 to 4 mice per group). **p < 0.01  , Mann-Whitney. (c) 16S bacterial rRNA sequencing was used to define the microbiota profiles of WT and Vα14 Tg littermate mice. These groups were separated by principal coordinates PCo1 and PCo2, collectively explaining 50.2% of the total similarity between samples, based on Bray-Curtis distances. 8 WT and 14 Vα14 Tg mice from 3 litters were analyzed. (d) Permutational multivariate analysis of the variance using Adonis. Data shows R² (effect size) and adjusted p values for genotype, sex, caging and parental group.