TABLE 2.

Susceptibilities of wild-type PAO1 and of omlA mutants to various detergents and antibiotics

Compounda	Zone of growth inhibition (mm)b
	PAO1	6B omlA::Tc	3A omlA::Tc
20% SDS	0	—c	13
10% Deoxycholate	0	10	13
5% CTAB	11	11	11
10% Triton X-100	14	14	14
10% Tween-20	0	0	0
0.5 M EDTA	15	15	15
2% Nalidixic acid	27	29	30
1% Rifampin	18	23	26
2% Novobiocin	19	21	24
2% Chloramphenicol	28	30	33
2% Cycloserine	10	10	10
1% Polymyxin B	22	22	22
1% Gentamicin	30	31	30

^aThe compounds were spotted (15 μl) on a sterile filter disk placed on M9 top agarose containing 10⁶ cells. 

^bThe zones were measured after 16 h at 37°C and are the averages for triplicate plates. 

^cPartial growth inhibition occurred in a zone of 12 mm.