Table 1. ANOVA results for Response Surface 2FI Model.
| Source | Sum of Squares | df | Mean Square | F Value | P value Prob > F | |
| Model | 2547.51 | 31 | 82.18 | 30.20 | ˂0.0001 | Significant |
| A-IPTG | 7.97 | 1 | 7.97 | 2.93 | 0.0935 | |
| B-OD600 | 101.26 | 1 | 101.26 | 37.21 | < 0.0001 | Significant |
| C-Induction Time | 425.12 | 4 | 106.28 | 39.06 | ˂0.0001 | Significant |
| D-Temperature | 1615.04 | 2 | 807.52 | 296.78 | < 0.0001 | Significant |
| AB | 1.70 | 1 | 1.70 | 0.63 | 0.4331 | |
| AC | 15.23 | 4 | 3.81 | 1.40 | 0.2484 | |
| AD | 1.10 | 2 | 0.55 | 0.2 | 0.8170 | |
| BC | 16.70 | 4 | 4.18 | 1.53 | 0.2071 | |
| BD | 19.02 | 2 | 9.51 | 3.50 | 0.0383 | Significant |
| CD | 255.92 | 8 | 31.99 | 11.76 | 0.0001 | Significant |
| A2 | 4.85 | 1 | 4.85 | 1.78 | 0.1883 | |
| B2 | 2.48 | 1 | 2.48 | 0.91 | 0.3446 | |
| Lack of Fit | 121.09 | 43 | 2.82 | 1.48 | 0.3557 | Not Significant |
| Residual | 130.61 | 48 | 0.13 | |||
| Pure Error | 9.51 | 5 | 1.90 | |||
| Cor Total | 2678.12 | 79 |