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| Induction time (h) | Temperature (°C) | Induction at OD 600 | IPTG (mM) | Volume productivity mg/L a | Specific production µg/mg b |
| 4 | 30 | 1.5 | 0.7 | 14.57 | 174.6 |
| 8 | 30 | 1.5 | 0.5 | 15.65 | 159.7 |
| 12 | 30 | 0.7 | 1 | 16.6 | 452.7 |
| 16 | 30 | 0.9 | 0.1 | 15.62 | 275.9 |
| 24 | 25 | 1.5 | 0.7 | 16.36 | 258.8 |
aCalculated based on ELISA assay and normalized to one liter of culture.
bCalculated by measuring the scFv concentration (determined by ELISA) per milligram of total protein fraction.
