Table 1. Primer sequences and characteristics .

Genes name	Gene Bank accession no	Sequences	Annealing temperature	Amplifying length
HIF-1α	NM_181054.2	HIF-1αF: 5’- CTTACACACAGAAATGGCCTTG-3’	60	133
		HIF-1αR: 5’- ATACCTTCCATGTTGCAGAC-3’
HIF-1β	NM_001197325.1	HIF-1β F: 5’- ACAATCATTCCCAGGTGGTTC-3’	61	148
		HIF-1β R: 5’- GCCTTTACTCTGATCCGCATTG-3’
Eno 1	NM_001428.3	Eno 1 F: 5’- GAGTCTCTTCAGGCGTGCAAG-3’	58	133
		Eno 1 R: 5’- CAGTCTTGATCTGCCCAGTGC -3’
GluT-1	NM_006516.2	GluT1 F: 5’- GTCTGGCATCAACGCTGTCTTC-3’	60	133
		GluT1 R: 5’- CACCACAAACAGCGACACGAC-3’
GAPDH	NM_002046.3	GAPF: 5’-AAGCTCATTTCCTGGTATGACAACG-3’	62	126
		GAPR: 5’-TCTTCCTCTTGTGCTCTTGCTGG-3’