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. 2022 May 6;67(2):241–252. doi: 10.1165/rcmb.2021-0563OC

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Copyright © 2022 by the American Thoracic Society

This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives License 4.0. For commercial usage and reprints, please e-mail Diane Gern.

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Figure 3. — AF^lo AMs share both AM- and monocyte-associated transcriptional signatures. (A) Heatmap depicting the list of significant DE genes jointly upregulated in AF^lo or AF^hi AMs as compared with blood monocytes. (B) Individual expression, shown as normalized counts, of the indicated genes within the indicated cell populations. (C) Heatmap depicting the list of significant DE genes commonly upregulated in blood monocytes or AF^lo AMs as compared with AF^hi AMs. (D) Individual expression, shown as normalized counts, of the indicated genes within the indicated cell populations. (E) Heatmap depicting the list of significant DE genes commonly upregulated in blood monocytes or AF^hi AMs as compared with AF^lo AMs. (F) CCR2 protein expression on BALF CD45⁺CD206⁺ macrophages (HD20 and HD21) (see Table E1). Representative flow cytometry plots showing CCR2 versus CD206 expression on BALF macrophages stained with (left) an anti-CCR2 or (center) FMO. Inserts indicated % of CCR2⁺ cells in the parent gate; (right) flow cytometry plots showing AF versus CD206 expression on total BALF CD45⁺ cells (gray) or CCR2⁺ macrophages (red). (B and D) P adjusted values are shown and were estimated thanks to the DESeq2 package. AF, auto-fluorescent; DE, differentially expressed; FMO = fluorescence minus one; HD = human donor; ns = nonsignificant. **P < 0.01 and ***P < 0.001.

Figure 3. — AF^lo AMs share both AM- and monocyte-associated transcriptional signatures. (A) Heatmap depicting the list of significant DE genes jointly upregulated in AF^lo or AF^hi AMs as compared with blood monocytes. (B) Individual expression, shown as normalized counts, of the indicated genes within the indicated cell populations. (C) Heatmap depicting the list of significant DE genes commonly upregulated in blood monocytes or AF^lo AMs as compared with AF^hi AMs. (D) Individual expression, shown as normalized counts, of the indicated genes within the indicated cell populations. (E) Heatmap depicting the list of significant DE genes commonly upregulated in blood monocytes or AF^hi AMs as compared with AF^lo AMs. (F) CCR2 protein expression on BALF CD45⁺CD206⁺ macrophages (HD20 and HD21) (see Table E1). Representative flow cytometry plots showing CCR2 versus CD206 expression on BALF macrophages stained with (left) an anti-CCR2 or (center) FMO. Inserts indicated % of CCR2⁺ cells in the parent gate; (right) flow cytometry plots showing AF versus CD206 expression on total BALF CD45⁺ cells (gray) or CCR2⁺ macrophages (red). (B and D) P adjusted values are shown and were estimated thanks to the DESeq2 package. AF, auto-fluorescent; DE, differentially expressed; FMO = fluorescence minus one; HD = human donor; ns = nonsignificant. **P < 0.01 and ***P < 0.001.