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. 2022 Apr 29;67(2):253–265. doi: 10.1165/rcmb.2021-0359OC

Figure 7.

Figure 7.

CF-HTBE cultures apically challenged with Pseudomonas aeruginosa exhibit mucin hypersecretion without alteration in macromolecular properties. (A and B) Representative images of whole-mount immunohistochemistry three-dimensional renderings of 120-hour tryptic soy broth (TSB) control (cont) (A) and P. aeruginosa (B) challenged CF-HTBE culture from the same donor. Blue: DAPI (nuclei). Red: MUC5AC. Green: MUC5B. (C) MUC5B and MUC5AC concentrations in HBE apical secretions after challenge as measured by label-free LC-MS/MS (n = 5). (D and E) Gel-forming mucins purified from apical secretions by isopycnic centrifugation show similar molecular weight (D) and radius of gyration (E) as measured by SEC-MALS (n = 7). (F and G) Ratio of unique MUC5B semitryptic peptides to fully tryptic peptides (F) and MUC5B semitryptic peptide localization is not significantly different between control and P. aeruginosa–challenged cultures (G). *P ⩽ 0.05.