Figure 1.

Overview of the experimental approach and findings in the alveolar macrophage (AM) pool (2). The identity and heterogeneity of alveolar macrophages were assessed among healthy nonsmokers, non-COPD smokers, and COPD smokers using a combination of approaches, including flow cytometry and bulk and single-cell RNA-seq of BAL cells. Illustration created with BioRender.com. AF^Hi = autofluorescent^hi; AF^Lo = autofluorescent^low; COPD = chronic obstructive pulmonary disease; FACS = fluorescence-activated cell sorting; sc-RNA Seq = small conditional RNA sequencing.