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. 2022 Jul 29;5(12):e202201424. doi: 10.26508/lsa.202201424

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© 2022 de Nola et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S3. — (A) AlphaScreen cross titration assay using MET-Fc chimera (0–10 nM) and K1K1H6 (0–300 nM) dilutions was performed using protein A–coated Alpha acceptor beads with Ni-NTA–coated Alpha donor beads. The Alpha signal is expressed in CPS (photon counts per seconds). Measurements are expressed as technical duplicates (mean ± SD, n = 2). (B) SPR analysis of K1K1 to immobilized MET receptor extracellular domain. The main plot shows the binding isotherm with equilibrium response plotted at different concentrations. The inserted graph shows the binding curves at different concentrations of K1K1. (C) SPR analysis of NK1 to immobilized MET receptor extracellular domain. As in (B), the main plot shows the binding isotherm and the inserted graph shows the binding curves at different concentrations of NK1.

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