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(A) NK1 and K1K1 were each injected at 2 and 1 μM on a heparin-coated SC HEP0320.a chip (Xantec). (B) Comparison of heparin affinity of K1K1, K1K1S2, and K1K1S4, injected at 2.5 and 1.25 μM each. All injections were done with a flow of 30 μl/min at 25°C in PBS running buffer.
