Table 1.

sgRNA sequences

sgRNA name	Sequence 5′–3′	Target gene
Bax_1_Fwd	caccgCAACTTCAACTGGGGCCGCG	Bax (mouse)
Bax_1_Rev	aaacCGCGGCCCCAGTTGAAGTTGc
Bax_2_Fwd	caccgAGCGAGTGTCTCCGGCGAAT
Bax_2_Rev	aaacATTCGCCGGAGACACTCGCTc
Bak_1_Fwd	caccGGAACTCTGTGTCGTAGCGC	Bak (mouse)
Bak_1_Rev	aaacGCGCTACGACACAGAGTTCC
Bbc3_1_Fwd	caccGCCGCTCGTACTGCGCGTTG	Bbc3 (mouse)
Bbc3_1_Rev	aaacCAACGCGCAGTACGAGCGGC
Bbc3_2_Fwd	caccGGGCACTCACCGTCCGGGCG
Bbc3_2_Rev	aaacCGCCCGGACGGTGAGTGCCC
Skp2_1_Fwd	caccgTTGCCCTTGACTCGTTTCCT	Skp2 (mouse)
Skp2_1_Rev	aaacAGGAAACGAGTCAAGGGCAAc
Skp2_2_Fwd	caccgTCCTTTATGGAGCAGCCGCT
Skp2_2_Rev	aaacAGCGGCTGCTCCATAAAGGAc
Cd8a_1_Fwd	caccGGTGTTGGGGTCCGTTTCGCA	Cd8a (mouse)
Cd8a_1_Rev	aaacTGCGAAACGGACCCCAACACC
Cd8a_2_Fwd	caccGGCTGGGTGAGTCGATTATCC
Cd8a_2_Rev	aaacGGATAATCGACTCACCCAGCC
SKP2‐1‐Fwd	caccgTCCCTCCAAAGGTGTTTCAT	SKP2 (human)
SKP2‐1‐Rev	aaacATGAAACACCTTTGGAGGGAc
SKP2‐2‐Fwd	caccgCCAGAGACCTTTAGCAGCTC
SKP2‐2‐Rev	aaacGAGCTGCTAAAGGTCTCTGGc