Fig. 1.

Knockdown of STAT3 expression induces membrane translocation of ICD‐related molecules in HCC cells and promotes DC activation. (A,B) Analysis of calreticulin⁺ and ERp57⁺ cells in Huh7 and HepG2.2.15 cells infected with indicated lentiviral vectors by flow cytometry. (C,D) Flow cytometry analysis of HSP70⁺ and HSP90⁺ cells in Huh7 and HepG2.2.15 cells. (E) Assessment of ATP released into culture supernatants of Huh7 and HepG2.2.15 cells. (F,G) Flow cytometry analysis of CD80⁺ and CD86⁺ cells in hDCs after coculture with Huh7 or HepG2.2.15 cells for 48 h. Data are shown as mean ± SD from three independent experiments and were analysed with unpaired Student's t‐test or one‐way ANOVA (*P < 0.05, **P < 0.01, and ***P < 0.001).