Figure S4. PFKFB4 depletion does not alter cell viability nor cell death.

(A) Cells (MeWo or A375M) were stained with 7-AAD and counted after siCo (blue curve) and siPFKFB4 (red curve) transfection. In both cell lines, cell death was minimal and the two conditions overlapped perfectly. Thus, PFKFB4 depletion did not increase cell death rate. (B) Similarly, the relative number of cells in each of the phase of the cell cycle was counted. In either cell line, global curves overlapped perfectly and the relative ratio of G1 (red), S (blue), and G2 (green) phase was unchanged. ## indicates difference between G2 and both G1 and S.