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. 2022 Aug 1;5(12):e202201377. doi: 10.26508/lsa.202201377

Figure S6. PFKFB4 and ICMT control AKT signaling in melanoma.

Figure S6.

(A) Control of the efficiency of siRNA targeting ICMT in MeWo cells. (B, C) Violin plots showing the travel distance (B) and pausing (C) parameters measured in MeWo cells, 48 h after transfection either with siControl, siPFKFB4, siICMT, or both. The violin plots represent the probability density at each value; lines are plotted at the median and quartiles. (D) Average speed of MeWo cells transfected with siRNA (siCtrl or siPFKFB4) and plasmid (empty vector or RasV12). (E) Protein levels pAKT S473, AKT, and actin in MeWo cells transfected with two different siRNA targeting PFKFB4. (F, G) Control of the efficiency of siRNAs targeting PFKFB4 in different melanoma cell lines by Western-blot 48 h after transfection. (H) Average speed of MeWo cells 48 h after transfection either with siControl+empty vector or siCtrl+caAKT. This experiment shows that caAKT alone does not increase the average speed of MeWo cells. (I) Average speed of A375M cells transfected either with siControl+empty vector, siPFKFB4+empty vector, siPFKFB4+xenopus PFKFB4 wild-type, and siPFKFB4+caAKT. (D, H, I) In (D, H, I): Graphs show the mean ± SEM. P-values were calculated using the Mann–Whitney test. n.s. P > 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.