Skip to main content
. 2022 Aug 1;5(12):e202201377. doi: 10.26508/lsa.202201377

Figure S7. PFKFB4 depletion affects AKT activation and cell migration in 12S2 mouse melanocytes.

Figure S7.

(A) Wild-type Mouse melanocytes (12S2) were used as a non-tumorigenic migratory cell type closely related to melanoma cells. Scale bar is 20 μm. (B) PFKFB4 was efficiently depleted using siRNA. (C) AKT phosphorylation was defective (S473) after PFKFB4 depletion. (D, E, F) Random single cell migration tracking showed that cell velocity was not significantly changed but that pausing time was significantly increased, resulting in a diminished total migrated distance. Graphs show the mean ± SEM. P-values were calculated using the Mann–Whitney test. n.s. P > 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.