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. 2022 Aug 3;17(8):e0271360. doi: 10.1371/journal.pone.0271360

Fig 1. Assessment of differentiation into astrocyte lineage.

Fig 1

Representative images of astrocytes differentiated from 3 healthy control donors and 2 donors with infantile onset Krabbe disease caused by a large deletion in the GALC gene. Cryopreserved astrocytes were thawed and cultured for 7 days prior to fixation and imaging. Immunocytochemistry revealed positive staining for astrocyte markers vimentin, s100β, CD44, and GLAST (EAAT1) in all cell lines used. Images were taken at 20x magnification.