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. 2022 Aug 3;13:944–959. doi: 10.18632/oncotarget.28261

Figure 1. LPS induces GLI3 expression in a HH independent manner.

Figure 1

(A) Human monocyte cell lines (MM6, THP-1, U937) (2 × 106 cells/ml) were treated with/without 100 ng/ml LPS (0111; B4 E.coli) for 1 h followed by determination of GLI expression by qPCR. (B) CD14+ cells from PBMCs (2 × 106 cells/ml) were stimulated with 100 ng/ml LPS for 1 h followed by qPCR to determine GLI3 expression. (C) MM6 cells (2 × 106 cells/ml) were stimulated with 100 ng/ml LPS for the indicated times, or with the indicated doses of LPS for 1 h followed by determination of GLI3 expression by qPCR. (D) Human monocytes (5 × 106 cells/ml) were stimulated with LPS for 12 h followed by determination of GLI3 protein expression by immunoblotting. All experiments were repeated at least three times. Data are presented as average of at least three independent experiments ± SEM.